Student Research: Resources

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  • Summer Research in the Sciences Summer Research in the Sciences

     

Resources for Research Students in Natural Sciences and Mathematics

Safety training and building access
Safety training is handled by the department faculty in collaboration with the chemical hygiene officer .  Building access may be available for students that pass safety training; consult with faculty and the chemical hygiene officer.
Trumbower: Dr. Joseph Keane and Mrs. Winnie Russell.
New Science Building and Shankweiler: Ms. Diane Dologite

Instrument training
Students may receive training to operate instruments. Which instruments are for public use is at the discretion of the faculty and the chemical hygiene officer.  Students may not operate an instrument without proper training.

Student researchers can find here a series of training videos for operating instrumentation and for conducting standard laboratory procedures.  These videos are not meant to replace personal training by a faculty member, but can serve as a reference for students when they are operating instruments independently.

Instrumentation and procedures pertaining to research in biochemistry, molecular and cell biology:

  • Beadbeater part 1 through part 4: This video demonstrates how to operate the BeadBeater from Biospec Products, from setup to cleanup.  The Beadbeater is a cell disruptor that uses  mechanical force to lyse cells open. The Beadbeater is a first step in protein extraction.
  • Centrifuge_part 1. This video explains how to balance tubes for high speed centrifugation.
  • Sorvall centrifuge_part 2. This video should be viewed along with “Centrifuge_part 1” when training to operate the Sorvall RC6 centrifuge. This 18 min video covers centrifuge operation and centrifuge safety. Students seeking a license to independently operate the Sorvall RC6 must first be trained by a faculty member. Once trained, a student can re-certify by watching this video and completing the quiz at the start of each new academic year.
  • NiNTA purification part 1 through part 3: This series of videos demonstrates a basic benchtop purification of a poly-histidine tagged protein using nickel affinity resin.
  • Peristaltic Pump in Purification: This video demonstrates how to add the peristaltic pump to your purification.
  • Desalting column: Why and how to use an Econo-Pac 10DG column (manufactured by BioRad) to buffer exchange a protein.
  • Allegra Centrifuge: This video should be viewed along with “Centrifuge Part 1” when preparing samples for centrifugation in the Allegra. The Allegra has a swinging bucket rotor and a maximum RCF of 5,200 x g – it is good for harvesting cell cultures. This centrifuge can spin 15mL(conical), 50mL (conical), 250mL (flat bottom) and 500mL (flat bottom) tubes.
  • Nano Drop: This video demonstrates how to operate the NanoDrop spectrometer for the purpose of measuring DNA concentration in a sample.
  • DNA Prep: This video demonstrates how to prep plasmid DNA from cells. You might use this video to prepare DNA for sequencing.
  • Preparing media to be autoclaved: This video demonstrates how to prepare flasks and bottles of liquid media for sterilization by the autoclave.
  • Pouring plates: This video demonstrates how to make sterile agar plates for bacterial growth.