Chemistry

Molecule Day 2004: DNA

 

Chemistry Recently (in 2003) there have been significant events in genomics: the completion of the Human Genome Project and the 50th Anniversary of the DNA double helix. To this end, this year’s interdisciplinary "Molecule of the Year" is DNA.

Seminar Speaker for the day was Dr. Robert Stanley of Temple University. His seminar entitled "Does DNA Flip for Photolyase?" was given followed by a poster session. A few of the titles from the poster session are listed.


  • Human centromere binding protein B
  • Site-specific recombinase XerD
  • DNA polymerase b
  • DNA ligase from bacteriophage T7
  • DNA cyclobutane dipyrimidine photolyase Anacystis nidulans and E. coli
  • DNA composition analysis by nuclease digestion and HPLC
  • The effect of UV irradiation on DNA using differential scanning calorimetry
  • EcoRI restriction endonuclease
  • Fluorescence measurements of ethidium and proflavin binding to DNA
  • Determination of the denaturation temperature of DNA with increasing ionic strength
  • Determination of binding parameters of ethidium bromide to DNA
  • DNA strand scission by H2O2 and Cu(II)bis-phenanthroline
  • Cloning and sequencing of a-tubulin gene from cultured Chinese hamster (cricetulus griseus) ovary cells
  • Cloning and sequencing of a TRPC1 gene from Chinese hamster ovary cells
  • Cloning and sequencing of a cytochrome c oxidase mitochondrial gene from a Chinese hamster ovary cell
  • Polyelectrolyte effects on ligand binding to nucleic acids
  • The thermodynamics of DNA duplex melting
  • Comparing the hyper-variable region of mitochondrial DNA between six individuals
  • Determination of relative concentrations of nucleic acids in insects
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